In 1835, he made the discovery of the germinal vesicle in the mammalian ovum, and in 1837, described the origin of the chorion.
2.
In GDF9 deficient oocytes the meiotic ability is significantly altered, where less than half will proceed metaphase 1 or 2 and a large percentage of oocytes have abnormal germinal vesicle breakdown.
3.
Besides that, in mouse oocytes, I-Carnitine ( LC ) supplementation during vitrification of germinal vesicle ( GV ) and their subsequent IVM improved nuclear maturation as well as meiotic spindle assembly and mitochondrial distribution in MII oocytes.
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